Difference between revisions of "Part:BBa K1649002"
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== Measurement == | == Measurement == | ||
| − | + | '''Design''' | |
<br> | <br> | ||
This part is a red light sensor combined with a tetR not logical gate followed by a GFP reporter. GFP can be used to evaluate the efficiency of red light sensor when this part is regulated by different promoters by measuring the florescence. In our experiment, the promoter is T7 promoter which is originally on pET28a and can be regulated by IPTG. In the preliminary experiment, we have searched out the suitable concentration of TPTG which are 0.6 mM and 0.8 mM. The sensor is inactive in dark and active under the red light. | This part is a red light sensor combined with a tetR not logical gate followed by a GFP reporter. GFP can be used to evaluate the efficiency of red light sensor when this part is regulated by different promoters by measuring the florescence. In our experiment, the promoter is T7 promoter which is originally on pET28a and can be regulated by IPTG. In the preliminary experiment, we have searched out the suitable concentration of TPTG which are 0.6 mM and 0.8 mM. The sensor is inactive in dark and active under the red light. | ||
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<br>'''Result''' | <br>'''Result''' | ||
<br> | <br> | ||
| − | From the statistics, we have found that 0.6 mM IPTG has a better induction, so the dates shown below are from 0.6 mM IPTG induced experiment. | + | From the statistics, we have found that 0.6 mM IPTG has a better induction, so the dates shown below are from 0.6 mM IPTG induced experiment. |
Revision as of 18:15, 20 September 2015
Red light sensor with TetR not gate and GFP reporter
As the transcription from the red light sensor is repressed by the function of red light and activated by dark, we use a not gate, repressor TetR and tetr operator to ensure the system is activated by red light.GFP can be used to test whether the circuit works.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 5318
Illegal XhoI site found at 382
Illegal XhoI site found at 2646 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4871
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 7067