Difference between revisions of "Part:BBa K1598005:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
  
−
This part consists of the pYeaR promoter: <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K216005">BBa_K216005</a>, an RBS, the TPH1 expression gene: [[BBa_K216005|Part:BBa_K216005|[https://parts.igem.org/wiki/index.php?title=Part:BBa_K216005]]] and a terminator.
+
This part consists of the pYeaR promoter: <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K216005">BBa_K216005</a>, an RBS, the TPH1 expression gene: Part:[[BBa_K1598001|https://parts.igem.org/wiki/index.php?title=Part:BBa_K1598001]] and a terminator.
  
 
- human TPH1 gene was codon-optimized for E.coli and provided with C-terminal 6xHis tag
 
- human TPH1 gene was codon-optimized for E.coli and provided with C-terminal 6xHis tag

Revision as of 17:04, 20 September 2015

pYear-RBS-TPH1-6xHis-Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part consists of the pYeaR promoter: <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K216005">BBa_K216005</a>, an RBS, the TPH1 expression gene: Part:https://parts.igem.org/wiki/index.php?title=Part:BBa_K1598001 and a terminator.

- human TPH1 gene was codon-optimized for E.coli and provided with C-terminal 6xHis tag - sequence was synthesized in the form of gBlock and cloned into pSB1C3

Source

DNA synthesis

References