Difference between revisions of "Part:BBa K1339024:Experience"

Revision as of 15:35, 20 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1339024

•••••

Tokyo-NoKoGen 2015 |width='60%' valign='top'| This part works well. The enzyme, G3DH, expressed from this parts could convert trehalose to 3'3-dkttrehalose. Following figure shows that G3DH can recognize trehalose as its substrate and it has activity toward trehalose. File:NK6.jpeg

Also, we built up one hypothesis that G3DH can recognize cellobiose as its substrate because of its wide specificity of substrate recognition. So, we checked the activity of G3DH towards cellobiose. Following figure shows that the G3DH has its activity towards cellobiose. File:Enz.jpeg From these results, Tokyo-NoKoGen 2015 figured out that the enzyme G3DH expressed from this parts has activity towards trehalose and cellobiose.

User Reviews

UNIQed6ccfbb0d8e941a-partinfo-00000001-QINU UNIQed6ccfbb0d8e941a-partinfo-00000002-QINU

@@ Line 5: / Line 5: @@
 ===Applications of BBa_K1339024===
+<partinfo>BBa_K1339005 AddReview 5</partinfo>
+<I>Tokyo-NoKoGen 2015</I>
+|width='60%' valign='top'|
+This part works well.
+The enzyme, G3DH,  expressed from this parts could convert trehalose to 3'3-dkttrehalose.
+Following figure shows that G3DH can recognize trehalose as its substrate and it has activity toward trehalose.
+[[File:NK6.jpeg]]
+Also, we built up one hypothesis that G3DH can recognize cellobiose as its substrate because of its wide specificity of substrate recognition.
+So, we checked the activity of G3DH towards cellobiose.
+Following figure shows that the G3DH has its activity towards cellobiose.
+[[File:Enz.jpeg]]
+From these results, Tokyo-NoKoGen 2015 figured out that the enzyme G3DH expressed from this parts has activity towards trehalose and cellobiose.
 ===User Reviews===