Difference between revisions of "Part:BBa K1614002:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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T7 promoter for expression
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The cassette contains a T7 promoter which enables the transcription of the RNA of Interest using the T7 RNA Polymerase. Cleavage of the RNA of Interest is achieved by adding an HDV behind the RNA of Interest.
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+
[[File:HeidelbergPWiGEM2015_RFC.png|thumb|General mechanism of the RFC which is based on BioBrick BBa_K1614002]]
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===Source===
 
===Source===

Revision as of 15:22, 20 September 2015


transcription cassette of BBF RFC 110


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 32
    Illegal NgoMIV site found at 61
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The cassette contains a T7 promoter which enables the transcription of the RNA of Interest using the T7 RNA Polymerase. Cleavage of the RNA of Interest is achieved by adding an HDV behind the RNA of Interest.

General mechanism of the RFC which is based on BioBrick BBa_K1614002

Source

Standard assembly construct for RFC 110 assembly

References