Difference between revisions of "Part:BBa K1614018"

Revision as of 14:17, 20 September 2015

ATP Aptamer Spinach 2.1

The ATP Aptamer Spinach2.1 is the first fluorescent ATP sensor that was designed for the in vitro transcription tool box designed by the iGEM Team Heidelberg 2015. This ATP Aptamer Spinach2.1 is cloned into the RFC 110.

Usage and Biology

ATP Aptamer Spinach 2.1 is a fusion of the BBa_K1330000 generated by DTU Danemark. According to our results, this fusion should contain the same properties as BBa_K1614012. Yet, this Spinach 2.1 is ligand dependend to ATP and should find its use in the detection of the small molecule ATP. Applications such as nucleotide sensing during in vitro transcription are possible. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 39
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1614018 short</partinfo>
-ATP Spinach
+The ATP Aptamer Spinach2.1 is the first fluorescent ATP sensor that was designed for the in vitro transcription tool box designed by the iGEM Team Heidelberg 2015. This ATP Aptamer Spinach2.1 is cloned into the RFC 110.
-<!-- Add more about the biology of this part here
+<!-- Add more about the biology of this part here -->
 ===Usage and Biology===
+ATP Aptamer Spinach 2.1 is a fusion of the BBa_K1330000 generated by DTU Danemark. According to our results, this fusion should contain the same properties as BBa_K1614012. Yet, this Spinach 2.1 is ligand dependend to ATP and should find its use in the detection of the small molecule ATP. Applications such as nucleotide sensing during in vitro transcription are possible.
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>