Difference between revisions of "Part:BBa K1614020"

Revision as of 14:14, 20 September 2015

ATP Aptamer JAWS2 Spinach 2.1

The ATP Aptamer JAWS 2.2 is cloned into the RFC 110 and can be transcribed using the T7 RNA Polymerase. This BioBrick can be used as a tool for sensing ATP in vivo.

Usage and Biology

ATP Aptamer JAWS2 Spinach 2.1 is a fusion of the BBa_K1330000 generated by DTU Danemark. According to our results, this fusion should contain similar properties as BBa_K1614014. Yet, this Spinach 2.1 is ligand dependend to ATP and should find its use in the detection of the small molecule ATP. Applications such as nucleotide sensing during in vitro transcription or because of its stronger stem for live cell imaging are possible. For more information check BBa_K1614014 or BBa_K1614015.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 47
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1614020 short</partinfo>
-ATP Spinach
+The ATP Aptamer JAWS 2.2 is cloned into the RFC 110 and can be transcribed using the T7 RNA Polymerase. This BioBrick can be used as a tool for sensing ATP in vivo.
 <!-- Add more about the biology of this part here -->
 ===Usage and Biology===
-ATP Aptamer JAWS2 Spinach 2.1 is a fusion of the BBa_K1330000 generated by DTU Danemark. According to our results, this fusion should contain similar properties as BBa_K1614014. Yet, this Spinach 2.1 is ligand dependend to ATP and should find its use in the detection of the small molecule ATP. Applications such as nucleotide sensing during in vitro transcription or because of its strong stem live cell imaging are possible. For more information check BBa_K1614014.
+ATP Aptamer JAWS2 Spinach 2.1 is a fusion of the BBa_K1330000 generated by DTU Danemark. According to our results, this fusion should contain similar properties as BBa_K1614014. Yet, this Spinach 2.1 is ligand dependend to ATP and should find its use in the detection of the small molecule ATP. Applications such as nucleotide sensing during in vitro transcription or because of its stronger stem for live cell imaging are possible. For more information check BBa_K1614014 or BBa_K1614015.
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