Difference between revisions of "Part:BBa K1614014"
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Created from Part:BBa_K1614011 to sense ATP with a fluorescent read out. | Created from Part:BBa_K1614011 to sense ATP with a fluorescent read out. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Another biobrick, the ATP Aptamer JAWS1 Spinach2 (BBa_K1614014) was designed in this project as well. Similar to the biobrick BBa_K1614012, we performed in vitro transcriptions to sense ATP in real-time. The construct is a fusion of an ATP aptamer (Sassanfar 1993) and Spinach2 (Strack 2013), which we will call Spinach2-ATP-Aptamer system . To improve the binding of the ATP aptamer to ATP we apply our own implemented JAWS software (Fig. 1). Using our software, nucleotides which form the stem region of the ATP aptamer can be predicted, which will improve binding properties of this RNA to ATP advanced the stemming behavior of the ATP Aptamer which was then fused to the Spinach2. | Another biobrick, the ATP Aptamer JAWS1 Spinach2 (BBa_K1614014) was designed in this project as well. Similar to the biobrick BBa_K1614012, we performed in vitro transcriptions to sense ATP in real-time. The construct is a fusion of an ATP aptamer (Sassanfar 1993) and Spinach2 (Strack 2013), which we will call Spinach2-ATP-Aptamer system . To improve the binding of the ATP aptamer to ATP we apply our own implemented JAWS software (Fig. 1). Using our software, nucleotides which form the stem region of the ATP aptamer can be predicted, which will improve binding properties of this RNA to ATP advanced the stemming behavior of the ATP Aptamer which was then fused to the Spinach2. | ||
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2.Strack, R.L. and S.R. Jaffrey, Live-cell imaging of mammalian RNAs with Spinach2. Methods Enzymol, 2015. 550: p. 129-46. | 2.Strack, R.L. and S.R. Jaffrey, Live-cell imaging of mammalian RNAs with Spinach2. Methods Enzymol, 2015. 550: p. 129-46. | ||
3. Strack, R.L., M.D. Disney, and S.R. Jaffrey, A superfolding Spinach2 reveals the dynamic nature of trinucleotide repeat-containing RNA. Nat Methods, 2013. 10(12): p. 1219-24. | 3. Strack, R.L., M.D. Disney, and S.R. Jaffrey, A superfolding Spinach2 reveals the dynamic nature of trinucleotide repeat-containing RNA. Nat Methods, 2013. 10(12): p. 1219-24. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 14:06, 20 September 2015
ATP Aptamer JAWS1 Spinach2
Created from Part:BBa_K1614011 to sense ATP with a fluorescent read out.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 165
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 165
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 77
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 165
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 165
Illegal NgoMIV site found at 178
Illegal NgoMIV site found at 207 - 1000COMPATIBLE WITH RFC[1000]