Difference between revisions of "Part:BBa K1486003"

Revision as of 12:22, 20 September 2015

Flexible linker 2x (GGGS)

Flexible linker used to fuse two proteins.

Sequence and Features

Assembly Compatibility:

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 Flexible linker used to fuse two proteins.
-===Applications of BBa_K1486003===
-'''Group''': iGEM15_Fudan<br>
-'''Author''': Xuhang Li <br>
-'''Summary''':We developed a new part(BBa_K1777005) based on this parts.When we planned to use this part to link our fusion component,we found it hard to design a proper primer to amplify it because of mutiple primer binding site as a result of tandem GGGS.Therefore, we first improved it by change the first GGGS to a natural linker obtained from linker database.In addition,the codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Such an improved GS linker could be easily assembled to your protein via overlap PCR and offer the property of nuclear localization.<br>
-<!-- Add more about the biology of this part here
 ===Usage and Biology===