Difference between revisions of "Part:BBa K1777016:Design"

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===Source===
 
===Source===
  
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We build this two device with [https://parts.igem.org/Part:BBa_K1777015 BBa_K1777015] and [https://parts.igem.org/Part:BBa_K1777003 BBa_K1777003], as well as beta-globin intron([https://parts.igem.org/Part:BBa_K404107 BBa_K404107]).
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We build this two device with [https://parts.igem.org/Part:BBa_K1777015 BBa_K1777015] and [https://parts.igem.org/Part:BBa_K1777003 BBa_K1777003], as well as beta-globin intron([https://parts.igem.org/Part:BBa_K404107 BBa_K404107]). We used the β-globin intron to increase the expression level of mRNA and provide SA and SD site to help the cirlization of mRNA at the same time.
  
 
===References===
 
===References===

Revision as of 11:51, 20 September 2015


Device to produce circRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 462
    Illegal XhoI site found at 833
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 804


Design Notes

This device is specially designed based on back-splicing mechanism.


Source

We build this two device with BBa_K1777015 and BBa_K1777003, as well as beta-globin intron(BBa_K404107). We used the β-globin intron to increase the expression level of mRNA and provide SA and SD site to help the cirlization of mRNA at the same time.

References