Difference between revisions of "Part:BBa K1680007"

 
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<partinfo>BBa_K1680007 short</partinfo>
 
<partinfo>BBa_K1680007 short</partinfo>
  
Protein coding region for the DNA recombinase Cre, codon optimized for yeast. The Cre recombinase recognizes two loxP sites (Part: BBa_K1680005) and depending on their orientation cuts out or reverses the DNA sequence between them.  
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This part contains the protein coding sequence for the DNA recombinase Cre and is codon optimized for yeast. Cre recombinases recognize two loxp sites ([[BBa_K1680005]]) and depending on their orientation cuts out or reverses the DNA sequence between them.
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
<partinfo>BBa_K1680007 SequenceAndFeatures</partinfo>
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<span class='h3bb'>This part is submitted in pSB1C3 with RFC25 standard. The part only sequence does therefore '''not''' contain a start and stop codon.</span>
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<partinfo>BBa_K1680006 SequenceAndFeatures</partinfo>
  
  

Revision as of 10:31, 20 September 2015

Cre recombinase

This part contains the protein coding sequence for the DNA recombinase Cre and is codon optimized for yeast. Cre recombinases recognize two loxp sites (BBa_K1680005) and depending on their orientation cuts out or reverses the DNA sequence between them.


Sequence and Features

This part is submitted in pSB1C3 with RFC25 standard. The part only sequence does therefore not contain a start and stop codon.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 268
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]