Difference between revisions of "Part:BBa K1631019:Experience"
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<p>We constructed new composite CheZ generators(CheZ strong1 generator [[Part:BBa_K1631018]], CheZ strong2 generator[[part:BBa_K1631019]], Medium CheZ generator[[part:BBa_K1631020]]) and introduced them to CheZ knockout strain named JW1870 (Keio collection). In contrast to previous iGEM projects, we tried to measure diffusion rate of <i>E.coli</i> quantitavely. In this end we took time lapse photography to measure the colony expanding speed. By combination with measurement of growth rate we could successfully quantitate diffusion rates according to different strength of CheZ | <p>We constructed new composite CheZ generators(CheZ strong1 generator [[Part:BBa_K1631018]], CheZ strong2 generator[[part:BBa_K1631019]], Medium CheZ generator[[part:BBa_K1631020]]) and introduced them to CheZ knockout strain named JW1870 (Keio collection). In contrast to previous iGEM projects, we tried to measure diffusion rate of <i>E.coli</i> quantitavely. In this end we took time lapse photography to measure the colony expanding speed. By combination with measurement of growth rate we could successfully quantitate diffusion rates according to different strength of CheZ | ||
expression. </p> | expression. </p> | ||
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| + | ===Applications of BBa_K1631019=== | ||
| + | ===User Reviews=== | ||
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Revision as of 08:56, 20 September 2015
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
We constructed new composite CheZ generators(CheZ strong1 generator Part:BBa_K1631018, CheZ strong2 generatorpart:BBa_K1631019, Medium CheZ generatorpart:BBa_K1631020) and introduced them to CheZ knockout strain named JW1870 (Keio collection). In contrast to previous iGEM projects, we tried to measure diffusion rate of E.coli quantitavely. In this end we took time lapse photography to measure the colony expanding speed. By combination with measurement of growth rate we could successfully quantitate diffusion rates according to different strength of CheZ expression.
Applications of BBa_K1631019
User Reviews
UNIQ008da236e61c1afc-partinfo-00000000-QINU UNIQ008da236e61c1afc-partinfo-00000001-QINU