Difference between revisions of "Part:BBa K1486003"
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Flexible linker used to fuse two proteins.
 
Flexible linker used to fuse two proteins.
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<br><br>improvements based on this protein<br>
 
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Group: iGEM15_Fudan<br>
 
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Author: Xuhang Li/n<br>
 
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Summary:We developed a new part(BBa_K1777005) based on this parts. The codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Besides ,we lengthen this linker to provide better separation of two domains. At last we avoid tandem repeat sequence(like GGGSPKKKRKVGGGS), which will facilitate fusing this linker to your domains via overlap PCR.
 
  
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===Applications of BBa_K1486003===
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'''Group''': iGEM15_Fudan<br>   
 +
'''Author''': Xuhang Li <br>       
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'''Summary''':We developed a new part(BBa_K1777005) based on this parts.When we planned to use this part to link our fusion component,we found it hard to design a proper primer to amplify it because of mutiple primer binding site as a result of tandem GGGS.Therefore, we first improved it by change the first GGGS to a natural linker obtained from linker database.In addition,the codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Such an improved GS linker could be easily assembled to your protein via overlap PCR and offer the property of nuclear localization.
 
<!-- Add more about the biology of this part here
 
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Revision as of 08:08, 20 September 2015

Flexible linker 2x (GGGS)

Flexible linker used to fuse two proteins.

Applications of BBa_K1486003

Group: iGEM15_Fudan
Author: Xuhang Li
Summary:We developed a new part(BBa_K1777005) based on this parts.When we planned to use this part to link our fusion component,we found it hard to design a proper primer to amplify it because of mutiple primer binding site as a result of tandem GGGS.Therefore, we first improved it by change the first GGGS to a natural linker obtained from linker database.In addition,the codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Such an improved GS linker could be easily assembled to your protein via overlap PCR and offer the property of nuclear localization. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]