Difference between revisions of "Part:BBa K1486003"
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Flexible linker used to fuse two proteins.
 
Flexible linker used to fuse two proteins.
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Group: iGEM15_Fudan<br>
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Author: Xuhang Li/n<br>
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Summary:We developed a new part(BBa_K1777005) based on this parts. The codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Besides ,we lengthen this linker to provide better separation of two domains. At last we avoid tandem repeat sequence(like GGGSPKKKRKVGGGS), which will facilitate fusing this linker to your domains via overlap PCR.
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Group: iGEM15_Fudan/n
 
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Author: Xuhang Li/n
 
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Summary:We developed a new part(BBa_K1777005) based on this parts. The codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Besides ,we lengthen this linker to provide better separation of two domains. At last we avoid tandem repeat sequence(like GGGSPKKKRKVGGGS), which will facilitate fusing this linker to your domains via overlap PCR.
 
 
===Usage and Biology===
 
===Usage and Biology===
  

Revision as of 08:02, 20 September 2015

Flexible linker 2x (GGGS)

Flexible linker used to fuse two proteins.
Group: iGEM15_Fudan
Author: Xuhang Li/n
Summary:We developed a new part(BBa_K1777005) based on this parts. The codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Besides ,we lengthen this linker to provide better separation of two domains. At last we avoid tandem repeat sequence(like GGGSPKKKRKVGGGS), which will facilitate fusing this linker to your domains via overlap PCR. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]