Difference between revisions of "Part:BBa K1486003:Experience"

Revision as of 07:42, 20 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1486003

Group: iGEM15_Fudan Author: Xuhang Li Summary:We developed a new part(BBa_K1777005) based on this parts.When we planned to use the part to link our fusion component,we found it hard to design a proper primer to amplify it because of mutiple primer binding site as a result of tandem GGGS.Therefore, we first improved it by change the first GGGS to a natural linker obtained from linker database.In addition,the codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Such a improved GS linker could be easily assembled to your protein via overlap PCR and offer the property of nuclear localization.

User Reviews

UNIQ144c99c4eb1f368d-partinfo-00000000-QINU UNIQ144c99c4eb1f368d-partinfo-00000001-QINU

@@ Line 6: / Line 6: @@
 Group: iGEM15_Fudan
 Author: Xuhang Li
-Summary:We developed a new part(BBa_K1777005) based on this parts. The codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Besides ,we lengthen this linker to provide better separation of two domains. At last we avoid tandem repeat sequence(like GGGSPKKKRKVGGGS), which will facilitate fusing this linker to your domains via overlap PCR.
+Summary:We developed a new part(BBa_K1777005) based on this parts.When we planned to use the part to link our fusion component,we found it hard to design a proper primer to amplify it because of mutiple primer binding site as a result of tandem GGGS.Therefore, we first improved it by change the first GGGS to a natural linker obtained from linker database.In addition,the codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Such a improved GS linker could be easily assembled to your protein via overlap PCR and offer the property of nuclear localization.
 ===User Reviews===