Difference between revisions of "Part:BBa K1648004:Design"
Fatfaatlai (Talk | contribs) (→Design Notes) |
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===Design Notes=== | ===Design Notes=== | ||
| − | In order to introduce protein of interest to the magnetosome membrane, we add mamC | + | In order to introduce protein of interest to the magnetosome membrane, we add mamC in the front of multiple cloning sites of pSB1C3. |
[[File:K1648004 history.png|800px]] | [[File:K1648004 history.png|800px]] | ||
Latest revision as of 06:04, 20 September 2015
Insertion Kit for Fusing Protein of Interest to Magnetosome Membrane
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2759
Illegal XbaI site found at 2765
Illegal SpeI site found at 1
Illegal PstI site found at 7 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2759
Illegal SpeI site found at 1
Illegal PstI site found at 7 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2759
Illegal XhoI site found at 1162
Illegal XhoI site found at 2188 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2759
Illegal XbaI site found at 2765
Illegal SpeI site found at 1
Illegal PstI site found at 7 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2759
Illegal XbaI site found at 2765
Illegal SpeI site found at 1
Illegal PstI site found at 7
Illegal NgoMIV site found at 2546
Illegal NgoMIV site found at 2636 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 2611
Design Notes
In order to introduce protein of interest to the magnetosome membrane, we add mamC in the front of multiple cloning sites of pSB1C3.
Source
Magnetospirillum gryphiswaldense MSR-1 v2, complete genome