Difference between revisions of "Part:BBa K1648004:Design"
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In order to introduce protein of interest to the magnetosome membrane, we add mamC to the pSB1C3. | In order to introduce protein of interest to the magnetosome membrane, we add mamC to the pSB1C3. | ||
| − | [[File:K1648004 history.png| | + | [[File:K1648004 history.png|800px]] |
===Source=== | ===Source=== | ||
Revision as of 06:02, 20 September 2015
Insertion Kit for Fusing Protein of Interest to Magnetosome Membrane
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2759
Illegal XbaI site found at 2765
Illegal SpeI site found at 1
Illegal PstI site found at 7 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2759
Illegal SpeI site found at 1
Illegal PstI site found at 7 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2759
Illegal XhoI site found at 1162
Illegal XhoI site found at 2188 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2759
Illegal XbaI site found at 2765
Illegal SpeI site found at 1
Illegal PstI site found at 7 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2759
Illegal XbaI site found at 2765
Illegal SpeI site found at 1
Illegal PstI site found at 7
Illegal NgoMIV site found at 2546
Illegal NgoMIV site found at 2636 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 2611
Design Notes
In order to introduce protein of interest to the magnetosome membrane, we add mamC to the pSB1C3.
Source
Magnetospirillum gryphiswaldense MSR-1 v2, complete genome