Difference between revisions of "Part:BBa K1777005:Design"
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===References===
 
===References===
 +
linker database(http://www.ibi.vu.nl/programs/linkerdbwww/）
 +
George RA. and Heringa J. (2002) An analysis of protein domain linkers: their classification and role in protein folding. Protein Engineering 15, 871-879

Revision as of 05:46, 20 September 2015

flexible linker with SV40 NLS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Besides ,we lengthen this linker to provide better separation of two domains. At last we avoid tandem repeat sequence(like GGGSPKKKRKVGGGS), which will facilitate fusing this linker to your domains via overlap PCR. We are sorry for that we accidentally fused two identical linker tandemly in one part because of using the inappropriate assembly protocol.However, we have already proved that such a fusion has no influence on using this part by our protocol.


Source

This part is improved from BBa_K1486003.

References

linker database(http://www.ibi.vu.nl/programs/linkerdbwww/） George RA. and Heringa J. (2002) An analysis of protein domain linkers: their classification and role in protein folding. Protein Engineering 15, 871-879