Difference between revisions of "Part:BBa K1648006:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K1648006=== | ===Applications of BBa_K1648006=== | ||
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| + | Consist of Insertion Kit([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1648004 K1648004]) and GFP-nanobody ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1648005 K1648005]) in order to fuse GFP-nanobody to a transmembrane protein on magnetosome, and express GFP-nanobody on magnetsome membrane for downstream applications. | ||
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| + | GFP-nanobody will be expressed on the magnetosome after transformation of this part into A. vinelandii genome via random integration. Magnetosome with GFP-nanobodies can be applied in studying the level of expression of fused protein in Insertion Kit([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1648004 K1648004]) simply by measuring fluorescence intensity of magnetosome incubated with GFP beforehand. | ||
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| + | The part is verified by double digestion(Fig 1.). | ||
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| + | [[File:NIKnano.jpg|400px]] | ||
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| + | Fig 1. Checking of recombinant Insertion Kit for Fusing Protein of Interest to Magnetosome Membrane (BBa_K1648004) using double digestion. L: DNA ladder. Lane 1-3: Insertion kit with GFP-nanobody (BBa_K1648006) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site. | ||
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===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 05:33, 20 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1648006
Consist of Insertion Kit(K1648004) and GFP-nanobody (K1648005) in order to fuse GFP-nanobody to a transmembrane protein on magnetosome, and express GFP-nanobody on magnetsome membrane for downstream applications.
GFP-nanobody will be expressed on the magnetosome after transformation of this part into A. vinelandii genome via random integration. Magnetosome with GFP-nanobodies can be applied in studying the level of expression of fused protein in Insertion Kit(K1648004) simply by measuring fluorescence intensity of magnetosome incubated with GFP beforehand.
The part is verified by double digestion(Fig 1.).
Fig 1. Checking of recombinant Insertion Kit for Fusing Protein of Interest to Magnetosome Membrane (BBa_K1648004) using double digestion. L: DNA ladder. Lane 1-3: Insertion kit with GFP-nanobody (BBa_K1648006) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site.
User Reviews
UNIQ1652d15d208f9962-partinfo-00000000-QINU UNIQ1652d15d208f9962-partinfo-00000001-QINU