Difference between revisions of "Part:BBa K1648005:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K1648005=== | ===Applications of BBa_K1648005=== | ||
| + | The GFP-nanobody is PCR from Anti-GFP Nanobody with IgG kappa signal peptide and IgG1 Fc domain [[https://parts.igem.org/wiki/index.php?title=Part:BBa_K929102 K929102]]. | ||
| + | [[File:PCR GFPnanobody.jpg|350px]] | ||
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| + | Figure 1. The photo of 1% agarose gel electrophoresis showing PCR products. | ||
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| + | L: DNA ladder. Lane 2: GFP-nanobody. | ||
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| + | This Part is added to Insertion Kit([https://parts.igem.org/Part:BBa_K1648004 K1648004]), forming a new Part([https://parts.igem.org/Part:BBa_K1648006 K1648006]), which is used to express GFP-nanobody on the membrane of magnetosome. | ||
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| + | This Part were verified by double digestion (Fig. 2) and sequencing. | ||
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| + | [[File:GFPnano-c.JPG|350px]] | ||
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| + | Figure 2. Checking of recombinant plasmid using double digestion. L: DNA ladder. Lane 1-3: GFP- nanobody (BBa_K1648005) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site. | ||
===User Reviews=== | ===User Reviews=== | ||
<!-- DON'T DELETE --><partinfo>BBa_K1648005 StartReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K1648005 StartReviews</partinfo> | ||
Latest revision as of 05:31, 20 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1648005
The GFP-nanobody is PCR from Anti-GFP Nanobody with IgG kappa signal peptide and IgG1 Fc domain [K929102].
Figure 1. The photo of 1% agarose gel electrophoresis showing PCR products.
L: DNA ladder. Lane 2: GFP-nanobody.
This Part is added to Insertion Kit(K1648004), forming a new Part(K1648006), which is used to express GFP-nanobody on the membrane of magnetosome.
This Part were verified by double digestion (Fig. 2) and sequencing.
Figure 2. Checking of recombinant plasmid using double digestion. L: DNA ladder. Lane 1-3: GFP- nanobody (BBa_K1648005) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site.
User Reviews
UNIQ8f30faffa2ef4e6d-partinfo-00000000-QINU UNIQ8f30faffa2ef4e6d-partinfo-00000001-QINU