Difference between revisions of "Part:BBa K1777005:Design"
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__NOTOC__ | __NOTOC__ | ||
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===Design Notes=== | ===Design Notes=== | ||
The codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Besides ,we lengthen this linker to provide better separation of two domains. At last we avoid tandem repeat sequence(like GGGSPKKKRKVGGGS), which will facilitate fusing this linker to your domains via overlap PCR. | The codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Besides ,we lengthen this linker to provide better separation of two domains. At last we avoid tandem repeat sequence(like GGGSPKKKRKVGGGS), which will facilitate fusing this linker to your domains via overlap PCR. | ||
| − | + | We are sorry for that we accidentally fused two identical linker tandemly in one part because of using the inappropriate assembly protocol.However, we have already proved that such a fusion has no influence on using this part by our protocol. | |
Revision as of 05:28, 20 September 2015
flexible linker with SV40 NLS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Besides ,we lengthen this linker to provide better separation of two domains. At last we avoid tandem repeat sequence(like GGGSPKKKRKVGGGS), which will facilitate fusing this linker to your domains via overlap PCR. We are sorry for that we accidentally fused two identical linker tandemly in one part because of using the inappropriate assembly protocol.However, we have already proved that such a fusion has no influence on using this part by our protocol.
Source
This part is improved from BBa_K1486003.