Difference between revisions of "Part:BBa K1648004:Experience"

Revision as of 05:21, 20 September 2015

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Applications of BBa_K1648004

Different genes can be added into this part at multiple restriction sites, so that different proteins will be expressed on the magnetosome after transformation of the part into A. vinelandii genome via random integration.

A GFP-nanobody(BBa_K1648005) is added to this part in order to express GFP-nanobody on the magnetosome. This new part is BBa_K1648006

This Part were verified by double digestion (Fig. 1).

Fig 1. The photo of 1% agarose gel electrophoresis showing PCR products of operons in Magnetosome Island (MAI). L: DNA ladder. Lane 1: mamHIEJKLMN. Lane 2: mamOPQRBSTU. Lane 3: mamPQRBSTU. Lane 4: mamYXZftsZ-like. Lane 5: mamGFDC. Lane 6: mamGFD. Lane 7: mms6.

RFP is inserted in Insertion Kit for biobrick standard. The Insertion kit with RFP is transformed in E. coli (DH5 alpha). Expression of RFP in Insertion is shown(Fig 2.).

Fig 2. Insertion kit with RFP(BBa_K1648004) is expressed in E. coli (DH5 alpha)

User Reviews

UNIQ0a39a0974aa2e187-partinfo-00000000-QINU UNIQ0a39a0974aa2e187-partinfo-00000001-QINU

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 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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 ===Applications of BBa_K1648004===
+Different genes can be added into this part at multiple restriction sites, so that different proteins will be expressed on the magnetosome after transformation of the part into <i>A. vinelandii</i> genome via <b>random integration</b>.
+A GFP-nanobody([https://parts.igem.org/Part:BBa_BBa_K1648005 BBa_K1648005]) is added to this part in order to express GFP-nanobody on the magnetosome. This new part is [https://parts.igem.org/Part:BBa_BBa_K1648006 BBa_K1648006]
+This Part were verified by double digestion (Fig. 1).
+[[File:K1648004gel.jpg]]
+Fig 1. The photo of 1% agarose gel electrophoresis showing PCR products of operons in Magnetosome Island (MAI). L: DNA ladder. Lane 1: mamHIEJKLMN. Lane 2: mamOPQRBSTU. Lane 3: mamPQRBSTU. Lane 4: mamYXZftsZ-like. Lane 5: mamGFDC. Lane 6: mamGFD. Lane 7: mms6.
+RFP is inserted in Insertion Kit for biobrick standard. The Insertion kit with RFP is transformed in E. coli (DH5 alpha).
+Expression of RFP in Insertion is shown(Fig 2.).
+[[File:20150919_021936.jpg|350px]]
+Fig 2. Insertion kit with RFP(BBa_K1648004) is expressed in E. coli (DH5 alpha)
 ===User Reviews===
 <!-- DON'T DELETE --><partinfo>BBa_K1648004 StartReviews</partinfo>