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| | + | [[File:K1648045_pcr.png|350px|thumb|right|PCR product of K1648045: The photo of 1% agarose gel electrophoresis. L: DNA ladder. Lane 1: PCR product of oprF encoding from the Azotobacter vinelandii strain DJ genome. |
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| | ===Applications of BBa_K1648045=== | | ===Applications of BBa_K1648045=== |
| − | An ongoing experiment will be carried out for the comparison between the transformed bacteria with [https://parts.igem.org/Part:BBa_K1648048 BBa_K1648048] for oprF from Azotobacter vinelandii [https://parts.igem.org/Part:BBa_K1648049 BBa_K1648049] for mutated oprF from Azotobacter vinelandii and [https://parts.igem.org/Part:BBa_K1648050 BBa_K1648050] for [https://parts.igem.org/Part:BBa_K1172501 BBa_K1172501] from Germany iGEM team. For comparison,an identical promoter, J13002, which is a constitutive promoter, is chosen to add before the gene in psb1C3 for constitutive expression of different OprF in bacteria.
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| − | [[File:K1648048_dd.png|350px|thumb|right|Double digestion of K1648048: L: DNA ladder. Lane 1-3: Recombination Template for J13002-oprF(BBa_K1648048) with double digestion cut at EcoR1 and PstI sites, with single digestion at Pst1 site, without digestion.]]
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| | ===User Reviews=== | | ===User Reviews=== |
Revision as of 04:55, 20 September 2015
PCR product of K1648045: The photo of 1% agarose gel electrophoresis. L: DNA ladder. Lane 1: PCR product of oprF encoding from the Azotobacter vinelandii strain DJ genome.
Applications of BBa_K1648045
User Reviews
UNIQ6367492fbe4c3703-partinfo-00000000-QINU
UNIQ6367492fbe4c3703-partinfo-00000001-QINU
