Difference between revisions of "Part:BBa K1765000:Experience"
(→‎Applications of BBa_K1765000)
 
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[[File:NKG3DH.jpg]]
 
[[File:NKG3DH.jpg]]
  
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We checked the expression of G3DH by SDS=Page.
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Following figure shows the result of SDS-Page. From this result, this biobrick parts can produce the three enzymes, G3DH and OtsA, OtsB correctly.
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Also, we checked the function of OtsA, OtsB. We use glucose as the substrate of these two enzymes.
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We checked whether OtsA, OtsB can produce trehalose from glucose by TLC.
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Following figure shows the result of the TLC. The result shows that the OtsA, OtsB expressed form this biobrick can convert glucose and produce trehalose.
  
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https://drive.google.com/file/d/0B3uuTFZgOUy6SWRYdDFkV0lGOGc/view?usp=sharing
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[[File:SDS-Page.jpg]]
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To produce 3,3’-diketotorehalose in the cell of <i>E. coli</i>, we constructed following Biobrick ( BBa_K1765000 ).
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This parts can express the enzyme G3DH from <i>Rhizobium radiobacter</i>, and also two additionally enzymes OtsA, OtsB from <i>E. coli</i>. The two enzymes OtsA and OtsB can convert glucose to trehalose, which can support the function of G3DH in the termite’s gut.<br>
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===User Reviews===
 
===User Reviews===

Latest revision as of 03:49, 20 September 2015

Applications of BBa_K1765000

BBa_K1765000 is coding three enzymes, G3DH, OtsA and OtsB. Two enzymes, OtsA and OtsB can convert glucose into trehalose. On the other hand, G3DH is can convert trehalose into 3'3- diketo trehalose. In the iGEM project of Tokyo-Nokogen 2015, we focused on the 3'3-diketo trehalose. We think 3'3-diketo trehalose can inhibit the activity of the enzyme, trehalase, which is essential for insects, such as termites.
NKG3DH.jpg

We checked the expression of G3DH by SDS=Page. Following figure shows the result of SDS-Page. From this result, this biobrick parts can produce the three enzymes, G3DH and OtsA, OtsB correctly. Also, we checked the function of OtsA, OtsB. We use glucose as the substrate of these two enzymes. We checked whether OtsA, OtsB can produce trehalose from glucose by TLC. Following figure shows the result of the TLC. The result shows that the OtsA, OtsB expressed form this biobrick can convert glucose and produce trehalose.

SDS-Page.jpg

User Reviews

UNIQ3f5d06176303c86c-partinfo-00000000-QINU UNIQ3f5d06176303c86c-partinfo-00000001-QINU