Difference between revisions of "Part:BBa K1765000:Experience"
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BBa_K1765000 is coding three enzymes, G3DH, OtsA and OtsB.
 
BBa_K1765000 is coding three enzymes, G3DH, OtsA and OtsB.
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<m3>　To produce 3,3’-diketotorehalose in the cell of <i>E. coli</i>, we constructed following Biobrick ( BBa_K1765000 ). This parts can express the enzyme G3DH from <i>Rhizobium radiobacter</i>, and also two additionally enzymes OtsA, OtsB from <i>E. coli</i>. The two enzymes OtsA and OtsB can convert glucose to trehalose, which can support the function of G3DH in the termite’s gut.<br>
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Two enzymes, OtsA and OtsB can convert glucose into trehalose. On the other hand, G3DH is can convert trehalose into 3'3- diketo trehalose.
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In the iGEM project of Tokyo-Nokogen 2015, we focused on the 3'3-diketo trehalose.
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We think 3'3-diketo trehalose can inhibit the activity of the enzyme, trehalase, which is essential for insects, such as termites.
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/Users/YU/Desktop/parts/G3DH-OtsA-OtsB.png
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To produce 3,3’-diketotorehalose in the cell of <i>E. coli</i>, we constructed following Biobrick ( BBa_K1765000 ).  
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This parts can express the enzyme G3DH from <i>Rhizobium radiobacter</i>, and also two additionally enzymes OtsA, OtsB from <i>E. coli</i>. The two enzymes OtsA and OtsB can convert glucose to trehalose, which can support the function of G3DH in the termite’s gut.<br>
  
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　<center><img src="https://static.igem.org/mediawiki/2015/9/91/NK4.jpeg" width="900px" height="400px"></center>
 
  
  

Revision as of 03:32, 20 September 2015

Applications of BBa_K1765000

BBa_K1765000 is coding three enzymes, G3DH, OtsA and OtsB. Two enzymes, OtsA and OtsB can convert glucose into trehalose. On the other hand, G3DH is can convert trehalose into 3'3- diketo trehalose. In the iGEM project of Tokyo-Nokogen 2015, we focused on the 3'3-diketo trehalose. We think 3'3-diketo trehalose can inhibit the activity of the enzyme, trehalase, which is essential for insects, such as termites.
/Users/YU/Desktop/parts/G3DH-OtsA-OtsB.png <>


To produce 3,3’-diketotorehalose in the cell of E. coli, we constructed following Biobrick ( BBa_K1765000 ). This parts can express the enzyme G3DH from Rhizobium radiobacter, and also two additionally enzymes OtsA, OtsB from E. coli. The two enzymes OtsA and OtsB can convert glucose to trehalose, which can support the function of G3DH in the termite’s gut.


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