Difference between revisions of "Part:BBa K1806003"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1806003 short</partinfo> | <partinfo>BBa_K1806003 short</partinfo> | ||
Designed to close lacO present systems in higher temperatures by producing lacI. | Designed to close lacO present systems in higher temperatures by producing lacI. | ||
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| + | == Cloning == | ||
| + | The composite part was ligated with the pSB1C3 cloning vector and then verified with Colony PCR. The part was tagged as G-Block 5 and sample 5-6 had the right base pair range. The part was then cut with the EcoR1 and Pst1 restriction enzymes. | ||
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| + | [[File:6003-1.png]] [[File:6003-2.png]] | ||
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| + | [[File:6003-3.png]] [[File:6003-4.png]] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 23:33, 19 September 2015
T7+ ibPB RNA Thermometer + lacI
Designed to close lacO present systems in higher temperatures by producing lacI.
Cloning
The composite part was ligated with the pSB1C3 cloning vector and then verified with Colony PCR. The part was tagged as G-Block 5 and sample 5-6 had the right base pair range. The part was then cut with the EcoR1 and Pst1 restriction enzymes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]