Difference between revisions of "Part:BBa J69511"
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Hi, we are from NTU-Singapore and we have made single basepair mutants of the RBS used in this reporter construct. | Hi, we are from NTU-Singapore and we have made single basepair mutants of the RBS used in this reporter construct. | ||
| − | 1AT denotes substitution of A to T. | + | 1AT denotes substitution of A to T at the first base pair position, so on so forth. Based on BBa_B0034, we mutated all 12 base pairs of the RBS by changing each base with the other three bases. |
Our library entry can be found from BBa_K1676037 to BBa_K1676072. | Our library entry can be found from BBa_K1676037 to BBa_K1676072. | ||
Revision as of 17:22, 19 September 2015
GFP reporter device - LacI repressible
Hi, we are from NTU-Singapore and we have made single basepair mutants of the RBS used in this reporter construct.
1AT denotes substitution of A to T at the first base pair position, so on so forth. Based on BBa_B0034, we mutated all 12 base pairs of the RBS by changing each base with the other three bases.
Our library entry can be found from BBa_K1676037 to BBa_K1676072.
Results of the mutant library:
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 725