Difference between revisions of "Part:BBa K1676070"

Latest revision as of 17:06, 19 September 2015

GFP Reporter with Mutant RBS 12AC

This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the twelfth base pair. The base pair A is substituted to C. The construct consist of the pLac promoter BBa_R0011, mutant RBS derive from BBa_B0034 , GFP BBa_E0040 and double terminator BBa_B0015.

The purpose of this construct is to characterize the mutant RBS in Shewanella oneidensis MR1 strain.

This reporter can also be derived from the existing construct, BBa_J69511.

The characterization is shown below,

Growth curve:

10%2C11%2C12gc.png

GFP Reading:

It is shown to have a fluorescence reading lower than the wild type RBS reporter, BBa_J69511.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 725

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1676070 short</partinfo>
-This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the twelvth base pair. The base pair A is substituted to C. The construct consist of the pLac promoter BBa_R0011, mutant RBS derive from BBa_B0034 , GFP BBa_E0040 and double terminator BBa_B0015.
+This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the twelfth base pair. The base pair A is substituted to C. The construct consist of the pLac promoter BBa_R0011, mutant RBS derive from BBa_B0034 , GFP BBa_E0040 and double terminator BBa_B0015.
 The purpose of this construct is to characterize the mutant RBS in Shewanella oneidensis MR1 strain.