Difference between revisions of "Part:BBa K1676070"

 
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<partinfo>BBa_K1676070 short</partinfo>
 
<partinfo>BBa_K1676070 short</partinfo>
  
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This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the second base pair. The TWELVETH base pair A is substituted to C. The construct consist of the pLac promoter BBa_R0011, mutant RBS, eGFP BBa_E0040 and double terminator BBa_B0015. The purpose of this construct is to characterise the mutant RBS in Shewanella Oneidensis MR1 strain.
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This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the twelvth base pair. The base pair A is substituted to C. The construct consist of the pLac promoter BBa_R0011, mutant RBS derive from BBa_B0034 , GFP BBa_E0040 and double terminator BBa_B0015.  
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The purpose of this construct is to characterize the mutant RBS in Shewanella oneidensis MR1 strain.  
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This reporter can also be derived from the existing construct, BBa_J69511.
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The characterization is shown below,
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Growth curve:
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https://static.igem.org/mediawiki/2015/1/1b/10%2C11%2C12gc.png
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GFP Reading:
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https://static.igem.org/mediawiki/2015/2/22/Gfp101112.png
  
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It is shown to have a fluorescence reading lower than the wild type RBS reporter, BBa_J69511.
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K1676070 parameters</partinfo>
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<partinfo>BBa_K16760670 parameters</partinfo>
 
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Revision as of 17:02, 19 September 2015

GFP Reporter with Mutant RBS 12AC

This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the twelvth base pair. The base pair A is substituted to C. The construct consist of the pLac promoter BBa_R0011, mutant RBS derive from BBa_B0034 , GFP BBa_E0040 and double terminator BBa_B0015.

The purpose of this construct is to characterize the mutant RBS in Shewanella oneidensis MR1 strain.

This reporter can also be derived from the existing construct, BBa_J69511.

The characterization is shown below,

Growth curve:

10%2C11%2C12gc.png

GFP Reading:

Gfp101112.png

It is shown to have a fluorescence reading lower than the wild type RBS reporter, BBa_J69511.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 725