Difference between revisions of "Part:BBa K1676067"
 
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<partinfo>BBa_K1676067 short</partinfo>
 
<partinfo>BBa_K1676067 short</partinfo>
  
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This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the eleventh base pair. The base pair A is substituted to C.
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This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the eleventh base pair. The base pair A is substituted to C. The construct consist of the pLac promoter BBa_R0011, mutant RBS derive from BBa_B0034 , GFP BBa_E0040 and double terminator BBa_B0015.  
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The construct consist of the pLac promoter BBa_R0011, mutant RBS derive from BBa_B0034 , GFP BBa_E0040 and double terminator BBa_B0015.  
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The purpose of this construct is to characterize the mutant RBS in Shewanella oneidensis MR1 strain.  
 
The purpose of this construct is to characterize the mutant RBS in Shewanella oneidensis MR1 strain.  
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https://static.igem.org/mediawiki/2015/2/22/Gfp101112.png
 
https://static.igem.org/mediawiki/2015/2/22/Gfp101112.png
  
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It is shown to have a fluorescence reading higher than the wild type RBS reporter, BBa_J69511.
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It is shown to have a fluorescence reading lower than the wild type RBS reporter, BBa_J69511.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 16:58, 19 September 2015

GFP Reporter with Mutant RBS 11AC

This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the eleventh base pair. The base pair A is substituted to C. The construct consist of the pLac promoter BBa_R0011, mutant RBS derive from BBa_B0034 , GFP BBa_E0040 and double terminator BBa_B0015.

The purpose of this construct is to characterize the mutant RBS in Shewanella oneidensis MR1 strain.

This reporter can also be derived from the existing construct, BBa_J69511.

The characterization is shown below,

Growth curve:

10%2C11%2C12gc.png

GFP Reading:

Gfp101112.png

It is shown to have a fluorescence reading lower than the wild type RBS reporter, BBa_J69511.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 725