Difference between revisions of "Part:BBa K1676066"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K1676066 short</partinfo>
+
<partinfo>BBa_K1676065 short</partinfo>
 +
 
 +
This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the tenth base pair. The base pair A is substituted to T.
 +
 +
The construct consist of the pLac promoter BBa_R0011, mutant RBS derive from BBa_B0034 , GFP BBa_E0040 and double terminator BBa_B0015.
 +
 
 +
The purpose of this construct is to characterize the mutant RBS in Shewanella oneidensis MR1 strain.
 +
 
 +
This reporter can also be derived from the existing construct, BBa_J69511.
 +
 
 +
The characterization is shown below,
 +
 
 +
Growth curve:
 +
 
 +
https://static.igem.org/mediawiki/2015/2/2a/108GC.png
 +
 
 +
GFP Reading:
  
−
This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the second base pair. The TENTH base pair A is substituted to T. The construct consist of the pLac promoter BBa_R0011, mutant RBS, eGFP BBa_E0040 and double terminator BBa_B0015. The purpose of this construct is to characterise the mutant RBS in Shewanella Oneidensis MR1 strain.
+
https://static.igem.org/mediawiki/2015/8/82/Gfp10%2C8.png
  
 +
It is shown to have a fluorescence reading higher than the wild type RBS reporter, BBa_J69511.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:55, 19 September 2015

GFP Reporter with Mutant RBS 10AG

This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the tenth base pair. The base pair A is substituted to T.

The construct consist of the pLac promoter BBa_R0011, mutant RBS derive from BBa_B0034 , GFP BBa_E0040 and double terminator BBa_B0015.

The purpose of this construct is to characterize the mutant RBS in Shewanella oneidensis MR1 strain.

This reporter can also be derived from the existing construct, BBa_J69511.

The characterization is shown below,

Growth curve:

108GC.png

GFP Reading:

Gfp10%2C8.png

It is shown to have a fluorescence reading higher than the wild type RBS reporter, BBa_J69511.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 725