Difference between revisions of "Part:BBa K554001:Experience"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
Line 5: Line 4:
  
 
===Applications of BBa_K554001===
 
===Applications of BBa_K554001===
 +
 +
<h4><b><span>BBa_K554001 Testing</span></b></h4>
 +
  <h4>The flhDC promoter <a href="https://parts.igem.org/Part:BBa_K554001"  target="_blank" alt="BBa_K554001">BBa_K554001</a> from the 2011 UNICAMP-EMSE Brazil iGEM team was what we needed.
 +
</h4>
 +
  <h4>
 +
    In order to test the promoter, we ligated the GFP reporter part, <a href="https://parts.igem.org/Part:BBa_I13504" target="_blank">BBa_I13504</a>, downstream of the promoter, transformed it in DH5-α and measured the fluorescence on a plate reader after being induced with varying concentrations of epinephrine at OD=0.1.
 +
  </h4>
 +
  <br>
 +
  <div class="figure">
 +
<a href="https://static.igem.org/mediawiki/2015/8/83/FlhDC_characterisation_corrected_to_0_epi1.png" target="_blank"><img class="figurepic" src="https://static.igem.org/mediawiki/2015/8/83/FlhDC_characterisation_corrected_to_0_epi1.png"/></a>
 +
<br>
 +
<p><strong>Figure 2.</strong> Fluorescence of DH5-α cultures when flhDC promoter was induced at varying concentrations of (-)-epinephrine.
 +
</p>
 +
  </div>
 +
<br><Br>
 +
  <h4>
 +
    Fig. 2 shows that at after around OD = 1, fluorescence becomes higher in the cultures containing epinephrine compared to the culture with no epinephrine. On another note, no large increase in fluorescence was seen from increasing the concentration of epinephrine beyond 5 μM,
 +
    showing that 5 μM epinephrine is enough to induce the promoter to maximum strength.
 +
  </h4>
  
 
===User Reviews===
 
===User Reviews===

Revision as of 16:35, 19 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K554001

BBa_K554001 Testing

The flhDC promoter <a href="https://parts.igem.org/Part:BBa_K554001" target="_blank" alt="BBa_K554001">BBa_K554001</a> from the 2011 UNICAMP-EMSE Brazil iGEM team was what we needed.

In order to test the promoter, we ligated the GFP reporter part, <a href="https://parts.igem.org/Part:BBa_I13504" target="_blank">BBa_I13504</a>, downstream of the promoter, transformed it in DH5-α and measured the fluorescence on a plate reader after being induced with varying concentrations of epinephrine at OD=0.1.

<a href="FlhDC_characterisation_corrected_to_0_epi1.png" target="_blank"><img class="figurepic" src="FlhDC_characterisation_corrected_to_0_epi1.png"/></a>

Figure 2. Fluorescence of DH5-α cultures when flhDC promoter was induced at varying concentrations of (-)-epinephrine.



Fig. 2 shows that at after around OD = 1, fluorescence becomes higher in the cultures containing epinephrine compared to the culture with no epinephrine. On another note, no large increase in fluorescence was seen from increasing the concentration of epinephrine beyond 5 μM, showing that 5 μM epinephrine is enough to induce the promoter to maximum strength.

User Reviews

UNIQ21e0a3b4ab800d6c-partinfo-00000000-QINU UNIQ21e0a3b4ab800d6c-partinfo-00000001-QINU