Difference between revisions of "Part:BBa K1676039"
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<partinfo>BBa_K1676039 short</partinfo> | <partinfo>BBa_K1676039 short</partinfo> | ||
| − | This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the first base pair. The | + | This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the first base pair. The base pair A is substituted to T. |
The construct consist of the pLac promoter BBa_R0011, mutant RBS derive from BBa_B0034 , GFP BBa_E0040 and double terminator BBa_B0015. | The construct consist of the pLac promoter BBa_R0011, mutant RBS derive from BBa_B0034 , GFP BBa_E0040 and double terminator BBa_B0015. | ||
Latest revision as of 15:14, 19 September 2015
GFP Reporter with Mutant RBS 1AT
This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the first base pair. The base pair A is substituted to T. The construct consist of the pLac promoter BBa_R0011, mutant RBS derive from BBa_B0034 , GFP BBa_E0040 and double terminator BBa_B0015.
The purpose of this construct is to characterize the mutant RBS in Shewanella oneidensis MR1 strain.
This reporter can also be derived from the existing construct, BBa_J69511.
The characterization is shown below,
Growth curve:
GFP Reading:
It is shown to have a fluorescence reading higher than the wild type RBS reporter, BBa_J69511.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 725