Difference between revisions of "Part:BBa K1758376"
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<h2>Biology</h2>
 
<h2>Biology</h2>
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<p>The reporter part was used to build a biosensor detecting ingredients of date rape drugs, namely GBL and GHB that also functions in cell free protein synthesis (CFPS) assay. The promoter part (T7) is inducible by rhamnose addition. The specific 5' untranslated region enhances translation. sfGFP was used as reporter protein. In presence of a the repressor <a href="https://parts.igem.org/Part:BBa_K1758370">BBa_K1758370</a> the reporter gene translation is inhibited. In presence of additional GHB/GHB the repression is resolved and the reporter gene is translated.</p>
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<p>The reporter part was used to build a biosensor detecting ingredients of date rape drugs, namely GBL and GHB that also functions in cell free protein synthesis (CFPS) assay. The promoter part (T7) is inducible by rhamnose addition. The following operator site from <i>Agrobacterium tumefaciens</i> is the binding site for the repressor BlcR. The specific 5' untranslated region enhances translation. sfGFP was used as reporter protein. In presence of a the repressor BlcR(<a href="https://parts.igem.org/Part:BBa_K1758370">BBa_K1758370</a>) the reporter gene translation is inhibited. In presence of additional GHB/GHB the repression is resolved and the reporter gene is translated.</p>
 
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Revision as of 04:59, 19 September 2015

GHB / GBL biosensor - reporter part

This part contains all features from BBa_K1758102 plus the blc-operator site described in [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4083662/ Pan et al. 2013]. BlcR (see K1758370) can bind to the operator site. For more detailed information, please be referred to K1758377

Biology

The reporter part was used to build a biosensor detecting ingredients of date rape drugs, namely GBL and GHB that also functions in cell free protein synthesis (CFPS) assay. The promoter part (T7) is inducible by rhamnose addition. The following operator site from Agrobacterium tumefaciens is the binding site for the repressor BlcR. The specific 5' untranslated region enhances translation. sfGFP was used as reporter protein. In presence of a the repressor BlcR(BBa_K1758370) the reporter gene translation is inhibited. In presence of additional GHB/GHB the repression is resolved and the reporter gene is translated.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 122