Difference between revisions of "Part:BBa K1648003"

Latest revision as of 04:20, 19 September 2015

Construct Map for K1648003

Recombination Template for mamXY,mamGC and mms Operons with Promotor and Terminator

Consist of a template for recombination of mamXY ,mamGC and mms operon(K1648001), a promotor & RBS(J13002) in front of each template and a double terminator(B0015) at the back of each template.

This part is designed for homologous recombination to introduce the large magnetosome forming operon into Azotobactor vinelandii.

This part is introduced into A. vinelandii genome via random integration before full operon sequences to be transformed into A. vinelandii.

This Part were verified by double digestion (Fig. 1) and sequencing.

Fig 1. Checking of recombinant plasmid using double digestion. L: DNA ladder. Lane 1-3: Recombination Template for mamXY, mamGC and mms Operons with Promotor and Terminator (BBa_K1648003) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1413
Illegal BamHI site found at 512
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 683
Illegal NgoMIV site found at 865
Illegal NgoMIV site found at 955
Illegal NgoMIV site found at 1363
Illegal AgeI site found at 566
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 550
Illegal BsaI site found at 649
Illegal BsaI site found at 930

@@ Line 7: / Line 7: @@
 This part is introduced into <i>A. vinelandii</i> genome via <b>random integration</b> before full operon sequences to be transformed into <i>A. vinelandii</i>.
+This Part were verified by double digestion (Fig. 1) and sequencing.
 [[File:K1648003gel.jpg|350px]]
 <br>
-Fig. Checking of recombinant plasmid using double digestion.
+Fig 1. Checking of recombinant plasmid using double digestion.
 L: DNA ladder.
 Lane 1-3: Recombination Template for mamXY, mamGC and mms Operons with Promotor and Terminator (BBa_K1648003) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site.