Difference between revisions of "Part:BBa K1648002"

Revision as of 04:02, 19 September 2015

Construct Map for K1648002

Recombination Template for mamAB Operon with Promotor and Terminator

Consist of a template for recombination of mamAB operon(K1648000), a promotor & RBS(J13002) in front of each template and a double terminator(B0015) at the back of each template.

This part is designed for homologous recombination to introduce the large magnetosome forming operon into Azotobactor vinelandii.

This Part were verified by double digestion (Fig. 1) and sequencing.

This part is introduced into A. vinelandii genome via random integration before full operon sequences to be transformed into A. vinelandii.
Fig 1. Checking of recombinant plasmid using double digestion. L: DNA ladder. Lane 1-3: Recombination Template for mamAB Operon with Promotor and Terminator (BBa_K1648002) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 855
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 371
Illegal AgeI site found at 890
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 616

@@ Line 5: / Line 5: @@
 This part is designed for <b>homologous recombination</b> to introduce the large magnetosome forming operon into <i>Azotobactor vinelandii</i>.
+This Part were verified by double digestion (Fig. 1) and sequencing.
 This part is introduced into <i>A. vinelandii</i> genome via <b>random integration</b> before full operon sequences to be transformed into <i>A. vinelandii</i>.
 [[File:K16480001GEL.jpg|400px]]
 <br>
-Fig. Checking of recombinant plasmid using double digestion.
+Fig 1. Checking of recombinant plasmid using double digestion.
 L: DNA ladder.
 Lane 1-3: Recombination Template for mamAB Operon with Promotor and Terminator (BBa_K1648002) without digestion, with single digestion at XbaI site, with double digestion cut at XbaI and PstI site.