Difference between revisions of "Part:BBa K1631018"

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===Usage and Biology===
 
===Usage and Biology===
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<p>We constructed new composite CheZ generators(CheZ strong1 generator [[Part:BBa_K1631018]], CheZ strong2 generator[[part:BBa_K1631019]], Medium CheZ generator [[part:BBa_K1631020]]) and introduced them to CheZ knockout strain named JW1870 (Keio collection). In contrast to previous iGEM projects, we tried to measure diffusion rate of <i>E.coli</i> quantitavely. In this end we took time lapse photography to measure the colony expanding speed. By combination with measurement of growth rate we could successfully quantitate diffusion rates according to different strength of CheZ
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<p>We constructed new composite CheZ generators(CheZ strong1 generator [[part:BBa_K1631018]], CheZ strong2 generator[[part:BBa_K1631019]], Medium CheZ generator [[part:BBa_K1631020]]) and introduced them to CheZ knockout strain named JW1870 (Keio collection). In contrast to previous iGEM projects, we tried to measure diffusion rate of <i>E.coli</i> quantitavely. In this end we took time lapse photography to measure the colony expanding speed. By combination with measurement of growth rate we could successfully quantitate diffusion rates according to different strength of CheZ
 
expression. </p>
 
expression. </p>
  

Latest revision as of 03:54, 19 September 2015

Pconst(Strong) -rbs- CheZ -d.term

Strong, constitutive generator of CheZ. We characteriazed Part:BBa_K629003 with this part!

Usage and Biology

We constructed new composite CheZ generators(CheZ strong1 generator part:BBa_K1631018, CheZ strong2 generatorpart:BBa_K1631019, Medium CheZ generator part:BBa_K1631020) and introduced them to CheZ knockout strain named JW1870 (Keio collection). In contrast to previous iGEM projects, we tried to measure diffusion rate of E.coli quantitavely. In this end we took time lapse photography to measure the colony expanding speed. By combination with measurement of growth rate we could successfully quantitate diffusion rates according to different strength of CheZ expression.

alt text


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]