Difference between revisions of "Part:BBa K1631018"
(→Usage and Biology) |
|||
| Line 7: | Line 7: | ||
<!-- Add more about the biology of this part here --> | <!-- Add more about the biology of this part here --> | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | <p>We constructed new composite CheZ generators(CheZ strong1 generator [[Part:BBa_K1631018]], CheZ strong2 generator[[part:BBa_K1631019]], Medium CheZ generator[[part:BBa_K1631020]]) and introduced them to CheZ knockout strain named JW1870 (Keio collection). In contrast to previous iGEM projects, we tried to measure diffusion rate of <i>E.coli</i> quantitavely. In this end we took time lapse photography to measure the colony expanding speed. By combination with measurement of growth rate we could successfully quantitate diffusion rates according to different strength of CheZ | + | <p>We constructed new composite CheZ generators(CheZ strong1 generator [[Part:BBa_K1631018]], CheZ strong2 generator[[part:BBa_K1631019]], Medium CheZ generator [[part:BBa_K1631020]]) and introduced them to CheZ knockout strain named JW1870 (Keio collection). In contrast to previous iGEM projects, we tried to measure diffusion rate of <i>E.coli</i> quantitavely. In this end we took time lapse photography to measure the colony expanding speed. By combination with measurement of growth rate we could successfully quantitate diffusion rates according to different strength of CheZ |
expression. </p> | expression. </p> | ||
Revision as of 03:54, 19 September 2015
Pconst(Strong) -rbs- CheZ -d.term
Strong, constitutive generator of CheZ. We characteriazed Part:BBa_K629003 with this part!
Usage and Biology
We constructed new composite CheZ generators(CheZ strong1 generator Part:BBa_K1631018, CheZ strong2 generatorpart:BBa_K1631019, Medium CheZ generator part:BBa_K1631020) and introduced them to CheZ knockout strain named JW1870 (Keio collection). In contrast to previous iGEM projects, we tried to measure diffusion rate of E.coli quantitavely. In this end we took time lapse photography to measure the colony expanding speed. By combination with measurement of growth rate we could successfully quantitate diffusion rates according to different strength of CheZ expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]