Difference between revisions of "Part:BBa K1682017"
(→Usage and Biology) |
|||
| Line 2: | Line 2: | ||
<partinfo>BBa_K1682017 short</partinfo> | <partinfo>BBa_K1682017 short</partinfo> | ||
| − | This composite part is consists of a L-arabinose inducible P<sub>araBAD</sub> with GFP reporter <partinfo>BBa_I13540</partinfo>, and an IPTG inducible P<sub>lac</sub> with RFP reporter [https://parts.igem.org/Part:BBa_K1682016 Part:BBa_K1682016]. | + | This composite part is consists of a L-arabinose inducible <i>P<sub>araBAD</sub></i> with GFP reporter <partinfo>BBa_I13540</partinfo>, and an IPTG inducible P<sub>lac</sub> with RFP reporter [https://parts.igem.org/Part:BBa_K1682016 Part:BBa_K1682016]. |
The part was designed to investigate the possible effect of parallel sensing within a single plasmid. | The part was designed to investigate the possible effect of parallel sensing within a single plasmid. | ||
Revision as of 03:52, 19 September 2015
ParaBAD-GFP-Plac-RFP
This composite part is consists of a L-arabinose inducible ParaBAD with GFP reporter BBa_I13540, and an IPTG inducible Plac with RFP reporter Part:BBa_K1682016. The part was designed to investigate the possible effect of parallel sensing within a single plasmid.
Mechanism
Figure 1. Schematic diagram of the inducible ParaBAD-GFP-Plac-mrfp construct. The system can sense L-arabinose to give GFP output or sense IPTG to give RFP output.
Usage and Biology
In order to investigate the effect of parallel sensing, the dose response ParaBAD-gfp in pSB3K3-BBa_K1682017 (double construct) was compared with that in pSB3K3-BBa_I13540 (single construct) in E. coli DH10B.
Figure 2. Response curve of ParaBAD-gfp in a single construct and double construct under varying L-arabinose concentration.The different blue curves indicate different induction level of the co-expressed Plac-mrfp<i> construct. At varying the induction level of the co-expressed <i>Plac-mrfp<i> construct seems to have an effect on the expression level of <i>ParaBAD-gfp<i> in a double construct. At a higher IPTG concentration, the maximum expression of <i>ParaBAD-gfp<i> decreases.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
Illegal NheI site found at 3185
Illegal NheI site found at 3208 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 4386
Illegal BamHI site found at 1144 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 2882
Illegal AgeI site found at 2994 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1880
Illegal SapI site found at 961