Difference between revisions of "Part:BBa K1631020"
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CheZ under strong, constitutive promoter BBa_J23102 | CheZ under strong, constitutive promoter BBa_J23102 | ||
| + | UT-Tokyo 2015 characterized Part:BBa_K629003 with this part ! | ||
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Latest revision as of 03:51, 19 September 2015
CheZ generator (under constitutive promoter BBa_J23102)
CheZ under strong, constitutive promoter BBa_J23102 UT-Tokyo 2015 characterized Part:BBa_K629003 with this part !
Usage and Biology
We constructed new composite CheZ generators(CheZ strong1 generator Part:BBa_K1631018, CheZ strong2 generatorpart:BBa_K1631019, Medium CheZ generatorpart:BBa_K1631020) and introduced them to CheZ knockout strain named JW1870 (Keio collection). In contrast to previous iGEM projects, we tried to measure diffusion rate of E.coli quantitavely. In this end we took time lapse photography to measure the colony expanding speed. By combination with measurement of growth rate we could successfully quantitate diffusion rates according to different strength of CheZ expression.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]