Difference between revisions of "Part:BBa K1682017"

Revision as of 03:50, 19 September 2015

P_araBAD-GFP-P_lac-RFP

This composite part is consists of a L-Arabinose inducible P_araBAD with GFP reporter BBa_I13540, and an IPTG inducible P_lac with RFP reporter Part:BBa_K1682016. The part was designed to investigate the posible effect of parallel sensing within a single plasmid.

Mechanism

Figure 1. Schematic diagram of the inducible P_araBAD-GFP-P_lac-mrfp construct. The system can sense L-arabinose to give GFP output or sense IPTG to give RFP output.

Usage and Biology

In order to investigate the effect of parallel sensing, the dose response P_araBAD-gfp in pSB3K3-BBa_K1682017 (double construct) was compared with that in pSB3K3-BBa_I13540 (single construct) in E. coli DH10B.

Parallel expression BBa K1682017 fig2.jpg

Figure 2. Response curve of P_araBAD-gfp in a single construct and double construct under varying L-arabinose concentration.The different blue curves indicate different induction level of the co-expressed P_lac-mrfp construct. At varying the induction level of the co-expressed P_lac-mrfp construct seems to have an effect on the expression level of P_araBAD-gfp in a double construct. At a higher IPTG concentration, the maximum expression of P_araBAD-gfp decreases.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]

12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1205
Illegal NheI site found at 3185
Illegal NheI site found at 3208

21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 4386
Illegal BamHI site found at 1144

23
COMPATIBLE WITH RFC[23]

25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 979
Illegal AgeI site found at 2882
Illegal AgeI site found at 2994

1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1880
Illegal SapI site found at 961

@@ Line 7: / Line 7: @@
 ==Mechanism==
 [[File:Parallel_expression_BBa_K1682017.jpg|700px|centre|]]
-'''Figure 1. Schematic diagram of the inducible P<sub>araBAD</sub>-GFP-P<sub>lac</sub>-mRFP construct.''' The system can sense L-Arabinose to give GFP output or sense IPTG to give RFP output.
+'''Figure 1. Schematic diagram of the inducible <i>P<sub>araBAD</sub>-GFP-P<sub>lac</sub>-mrfp</i> construct.''' The system can sense L-arabinose to give GFP output or sense IPTG to give RFP output.
 ==Usage and Biology==
-In order to investigate the effect of parallel sensing, the dose response P<sub>araBAD</sub>-GFP in pSB3K3-<partinfo>BBa_K1682017</partinfo> (double construct) was compared with that in pSB3K3-<partinfo>BBa_I13540</partinfo> (single construct) in <i>E. coli</i> DH10B.
+In order to investigate the effect of parallel sensing, the dose response <i>P<sub>araBAD</sub>-gfp<i> in pSB3K3-<partinfo>BBa_K1682017</partinfo> (double construct) was compared with that in pSB3K3-<partinfo>BBa_I13540</partinfo> (single construct) in <i>E. coli</i> DH10B.
 [[File:Parallel_expression_BBa_K1682017_fig2.jpg|800px|centre|]]
-'''Figure 2. Response curve of P<sub>araBAD</sub>-GFP in a single construct and double construct under varying L-Arabinose concentration.'''The different blue curves indicate different induction level of the co-expressed PlacZ-mRFP construct. At varying the induction level of the co-expressed P<sub>lac</sub>-mRFP construct seems to have an effect on the expression level of P<sub>araBAD</sub>-GFP in a double construct. At a higher IPTG concentration, the maximum expression of P<sub>araBAD</sub>-GFP decreases.
+'''Figure 2. Response curve of <i>P<sub>araBAD</sub>-gfp</i> in a single construct and double construct under varying L-arabinose concentration.'''The different blue curves indicate different induction level of the co-expressed <i>P<sub>lac</sub>-mrfp<i> construct. At varying the induction level of the co-expressed <i>P<sub>lac</sub>-mrfp<i> construct seems to have an effect on the expression level of <i>P<sub>araBAD</sub>-gfp<i> in a double construct. At a higher IPTG concentration, the maximum expression of <i>P<sub>araBAD</sub>-gfp<i> decreases.