Difference between revisions of "Part:BBa K1611001"

 
 
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<partinfo>BBa_K1611001 short</partinfo>
 
<partinfo>BBa_K1611001 short</partinfo>
  
 
Murine IFN gamma cytokine codon optimized to give the best yield in yeast S. cerevisiae. It promotes T cell activation, upregulates MHC-I and is pro-inflammatory. MAT alpha is the peptide leader used as a secretion signal and fused to IFN gamma without ATG.
 
Murine IFN gamma cytokine codon optimized to give the best yield in yeast S. cerevisiae. It promotes T cell activation, upregulates MHC-I and is pro-inflammatory. MAT alpha is the peptide leader used as a secretion signal and fused to IFN gamma without ATG.
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<br>
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<h2>Part sequencing</h2>
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https://static.igem.org/mediawiki/parts/3/32/S%C3%A9quence_matalpha-IFNg.jpg
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<p class="text-justify"><strong> Figure 1: Sequencing of MATalpha-IFNgamma cloned in pSB1C3 vector. The black line shows the consensus sequence.</strong> </p>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:50, 19 September 2015

MATalpha-IFNgamma

Murine IFN gamma cytokine codon optimized to give the best yield in yeast S. cerevisiae. It promotes T cell activation, upregulates MHC-I and is pro-inflammatory. MAT alpha is the peptide leader used as a secretion signal and fused to IFN gamma without ATG.

Part sequencing

S%C3%A9quence_matalpha-IFNg.jpg

Figure 1: Sequencing of MATalpha-IFNgamma cloned in pSB1C3 vector. The black line shows the consensus sequence.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 244
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 297