Difference between revisions of "Part:BBa K1631019"

Revision as of 03:27, 19 September 2015

Pconst(medium) -rbs- cheZ -d.term

Constitutive CheZ generator (medium).

Usage and Biology

We constructed new composite CheZ generators(CheZ strong1 generator Part:BBa_K1631018, CheZ strong2 generatorpart:BBa_K1631019, Medium CheZ generatorpart:BBa_K1631020) and introduced them to CheZ knockout strain named JW1870 (Keio collection). In contrast to previous iGEM projects, we tried to measure diffusion rate of E.coli quantitavely. In this end we took time lapse photography to measure the colony expanding speed. By combination with measurement of growth rate we could successfully quantitate diffusion rates according to different strength of CheZ expression.

alt text

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1631019 short</partinfo>
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-<!-- Add more about the biology of this part here
+<!-- Add more about the biology of this part here -->
 ===Usage and Biology===
+<p>We constructed new composite CheZ generators(CheZ strong1 generator [[Part:BBa_K1631018]], CheZ strong2 generator[[part:BBa_K1631019]], Medium CheZ generator[[part:BBa_K1631020]]) and introduced them to CheZ knockout strain named JW1870 (Keio collection). In contrast to previous iGEM projects, we tried to measure diffusion rate of <i>E.coli</i> quantitavely. In this end we took time lapse photography to measure the colony expanding speed. By combination with measurement of growth rate we could successfully quantitate diffusion rates according to different strength of CheZ
+expression. </p>
+[[File:Nakamura coef of diffusion.png|600px|thumb|center|alt text]]
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