Difference between revisions of "Part:BBa K1758321:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K1758321 short</partinfo>
 
<partinfo>BBa_K1758321 short</partinfo>
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===Design Notes===
 
===Design Notes===
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We used the codon optimized, synthesized Sequence of RFP under the control of <i>copAP </i> from <i> E.coli </i> K12. We designed the used primes, in specially split primers, for Gibson Assembly to ligate the RFP under control of <i>copAP</i>  with pSB1C3. For this aim we designed four primers for the generation of homologous overlaps between the synthesized RFP under control of <i> copAP</i>  and the pSB1C3:
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<p><a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#cm_fwd" target="_blank"> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#cm_fwd" target="_blank">cm_fwd</a></p>
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<p>CGGCATCAGCACCTTGTC </p>
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<p><a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers# pSB1C3_RFP_rev" target="_blank"> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers# pSB1C3_RFP_rev" target="_blank"> pSB1C3_RFP_rev</a> </p>
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<p> CCGCCACTCAACGGGTGCCTGATAATACTAGTAGCGGCCGCTGCA </p>
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<p><a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#cm_rev" target="_blank"> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#cm_rev" target="_blank">cm_rev</a> </p>
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<p> TATACGCAAGGCGACAAG </p>
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<p><a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers#pSB1C3_copAP_fwd" target="_blank"> <a href="http://2015.igem.org/Team:Bielefeld-CeBiTec/Primers# pSB1C3_copAP_fwd" target="_blank"> pSB1C3_copAP_fwd</a></p>
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<p>TTGACCTTCCCCTTGCTGGAAGGTTTAACCTTTATCACAATTAAAGAGGAGAAATACTAG</p>
  
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===Source===
 
  
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CopAp is a copper induceble promoter out of ``E.coli`` K12
 
  
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===Source===
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<i>CopAp</i> is a copper induceble promoter out of <i>E.coli</i> K12
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===References===
 
===References===

Revision as of 03:09, 19 September 2015

mRFP under control of copper responsive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used the codon optimized, synthesized Sequence of RFP under the control of copAP from E.coli K12. We designed the used primes, in specially split primers, for Gibson Assembly to ligate the RFP under control of copAP with pSB1C3. For this aim we designed four primers for the generation of homologous overlaps between the synthesized RFP under control of copAP and the pSB1C3:

cm_fwd

CGGCATCAGCACCTTGTC

pSB1C3_RFP_rev

CCGCCACTCAACGGGTGCCTGATAATACTAGTAGCGGCCGCTGCA

cm_rev

TATACGCAAGGCGACAAG

pSB1C3_copAP_fwd

TTGACCTTCCCCTTGCTGGAAGGTTTAACCTTTATCACAATTAAAGAGGAGAAATACTAG



Source

CopAp is a copper induceble promoter out of E.coli K12

References