Difference between revisions of "Part:BBa K1758320"
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<partinfo>BBa_K1758320 short</partinfo> | <partinfo>BBa_K1758320 short</partinfo> | ||
| − | Activator for copper induceble promoter | + | Activator for copper induceble promoter <i>copAP</i> under the control of constitutive promoter (K608002) |
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | <html> | + | <html> <i>cueR</i> is a <i>merR</i> like regulator, which stimulates the transcription of <i>copAP</i>, a P-type ATPase pump (Outten et al. 2000). <i>CopAP</i> is the central component in obtaining copper homeostasis, it exports free copper from cytoplasm to the periplasm. This is enabled by copper induced activation of the operon transcription via CueR. The CueR-Cu+ is the DNA-binding transcriptional dual regulator which activates transcription (Yamamoto, Ishihama 2005).</html> |
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 03:05, 19 September 2015
copper activator under control constitutive promoter and strong RBS
Activator for copper induceble promoter copAP under the control of constitutive promoter (K608002)
Usage and Biology
cueR is a merR like regulator, which stimulates the transcription of copAP, a P-type ATPase pump (Outten et al. 2000). CopAP is the central component in obtaining copper homeostasis, it exports free copper from cytoplasm to the periplasm. This is enabled by copper induced activation of the operon transcription via CueR. The CueR-Cu+ is the DNA-binding transcriptional dual regulator which activates transcription (Yamamoto, Ishihama 2005).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]