Difference between revisions of "Part:BBa K1648006"

Revision as of 03:01, 19 September 2015

Insertion kit with GFP-nanobody

Consist of Insertion Kit(K1648004) and GFP-nanobody (K1648005) in order to fuse GFP-nanobody to a transmembrane protein on magnetosome, and express GFP-nanobody on magnetsome membrane for downstream applications.

GFP-nanobody will be expressed on the magnetosome after transformation of this part into A. vinelandii genome via random integration. Magnetosome with GFP-nanobodies can be applied in studying the characterization of level of expression of fused protein in Insertion Kit(K1648004) simply by measuring fluorescence intensity of magnetosome incubated with GFP beforehand.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1648006 short</partinfo>
-It contains a Consist of a genome (mamC) from magnetosome island of Magnetospirillum gryphiswaldense genome and GFP nanobody in order to fuse GFP nanobody to the membrane of magnetosome.
+Consist of Insertion Kit([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1648004 K1648004]) and GFP-nanobody ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1648005 K1648005]) in order to fuse GFP-nanobody to a transmembrane protein on magnetosome, and express GFP-nanobody on magnetsome membrane for downstream applications.
+GFP-nanobody will be expressed on the magnetosome after transformation of this part into A. vinelandii genome via random integration. Magnetosome with GFP-nanobodies can be applied in studying the characterization of level of expression of fused protein in Insertion Kit([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1648004 K1648004]) simply by measuring fluorescence intensity of magnetosome incubated with GFP beforehand.
 <!-- Add more about the biology of this part here