Difference between revisions of "Part:BBa K1595024:Experience"
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<h3>Overview of Characterization of MBP+EcnB BioBrick</h3>
 
<h3>Overview of Characterization of MBP+EcnB BioBrick</h3>
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As described on the <a href="http://2015.igem.org/Team:Cornell/wetlab">2015 Cornell iGEM wiki</a> section, the peptide EcnB is capable of inhibiting the growth of <i>F. psychrophillum</i> in order to treat bacterial coldwater disease prevalent in salmonid species. In order to stabilize the EcnB peptide, we have placed EcnB downstream of MBP, a maltose binding protein.
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As described on the Cornell iGEM wiki, the peptide EcnB is capable of inhibiting the growth of <i>F. psychrophillum</i> in order to treat bacterial coldwater disease prevalent in salmonid species. In order to stabilize the EcnB peptide, we have placed EcnB downstream of MBP, a maltose binding protein. We ran a series of disk diffusion assays from lysate from BBa_K1595024 as compared to antibiotics used in the field (oxytetracycline) and BL21 lysate as a negative control. <br>

Revision as of 02:53, 19 September 2015

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UNIQ9280802453f202af-partinfo-00000000-QINU UNIQ9280802453f202af-partinfo-00000001-QINU

Overview of Characterization of MBP+EcnB BioBrick

As described on the Cornell iGEM wiki, the peptide EcnB is capable of inhibiting the growth of F. psychrophillum in order to treat bacterial coldwater disease prevalent in salmonid species. In order to stabilize the EcnB peptide, we have placed EcnB downstream of MBP, a maltose binding protein. We ran a series of disk diffusion assays from lysate from BBa_K1595024 as compared to antibiotics used in the field (oxytetracycline) and BL21 lysate as a negative control.