Difference between revisions of "Part:BBa K1692000"

Revision as of 02:51, 19 September 2015

Ferulic acid decarboxylase (FDC)

Ferulic Acid Decarboxylase

Ferulic acid decarboxylase (FDC) catalyzes the conversion of trans-cinnamic acid to styrene. We isolated this genetic part from Saccharomyces cerevisiae and inserted the protein-coding sequence into the pSB1C3 backbone. The original sequence in yeast contains an SpeI restriction site in the 999th nucleotide position. Thus, we performed site-directed mutagenesis in order to make our part BioBrick compatible. Gene sequencing analysis confirmed that our site-directed mutagenesis was successful. See our <a href="https://parts.igem.org/Part:BBa_K1692002">"synthesized FDC"</a> for more results.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 760

@@ Line 6: / Line 6: @@
 <P>Ferulic acid decarboxylase (FDC) catalyzes the conversion of trans-cinnamic acid to styrene. We isolated this genetic part from Saccharomyces cerevisiae and inserted the protein-coding sequence into the pSB1C3 backbone. The original sequence in yeast contains an SpeI restriction site in the 999th nucleotide position. Thus, we performed site-directed mutagenesis in order to make our part BioBrick compatible. Gene sequencing analysis confirmed that our site-directed mutagenesis was successful.
-See our <a href="https://parts.igem.org/Part:BBa_K1692002">synthesized FDC</a> for more results.
+See our <a href="https://parts.igem.org/Part:BBa_K1692002">"synthesized FDC"</a> for more results.
 </P>