Difference between revisions of "Part:BBa K1734010"

 
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<partinfo>BBa_K1734010 short</partinfo>
 
<partinfo>BBa_K1734010 short</partinfo>
  
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c4
 
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===Usage and Biology===
 
===Usage and Biology===
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This construct contains the polyhedrin promoter, the Chicken Lysozyme signal peptide, Nanoluc coding sequence with a Histidine Tag, united with the T2A sequence for polycistronic expression, and the RFP sequence with a Histidine Tag.
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Signal peptide sequences are for extracellular secretion of Nanoluc, RFP remains inside the cell, this to compare extracellular and intracellular protein expression and characterize the functionality of the promoter.
  
 
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Revision as of 02:48, 19 September 2015

C4 (pPH +CL signal peptide + Nanoluc + T2A sequence + mRFP1 + 6xHis)

Usage and Biology

This construct contains the polyhedrin promoter, the Chicken Lysozyme signal peptide, Nanoluc coding sequence with a Histidine Tag, united with the T2A sequence for polycistronic expression, and the RFP sequence with a Histidine Tag.

Signal peptide sequences are for extracellular secretion of Nanoluc, RFP remains inside the cell, this to compare extracellular and intracellular protein expression and characterize the functionality of the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 690
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 614