Difference between revisions of "Part:BBa K1734007"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | This construct consists of a promoter p10, the Honeybee Melittin signal peptide, and the Nanoluc coding sequence with a Histidine Tag, a T2A sequence for polycistronic expression, and and finally RFP protein with Histidine Tag. | ||
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| + | Signal peptide sequences are for extracellular secretion of Nanoluc, RFP remains inside the cell, this to compare extracellular and intracellular protein expression and characterize the functionality of the promoter. | ||
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Revision as of 02:41, 19 September 2015
C1 (p10 + HBM signal sequence + Nanoluc + T2A sequence + mRFP1 + 6xHis)
C1 description
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 717
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 5
Illegal SapI site found at 641