Difference between revisions of "Part:BBa K1760001:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
−
This design contains;
+
This design contains: a constitutive TetO promoter without TetR gene, an RBS designed by using Salis RBS calculator and a coding todE sequence codon optimized for E.Coli
−
- a constitutive TetO promoter without TetR gene
+
−
- an RBS designed by using Salis RBS calculator
+
−
- a coding todE sequence codon optimized for E.Coli
+
  
 
This design is Gibson Assembled.
 
This design is Gibson Assembled.

Latest revision as of 02:36, 19 September 2015


TodE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 997
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This design contains: a constitutive TetO promoter without TetR gene, an RBS designed by using Salis RBS calculator and a coding todE sequence codon optimized for E.Coli

This design is Gibson Assembled.

Source

Pseudomonas putida F1 (Synthesized)

References